Fluorescence:Article Title: Short RNA chaperones promote aggregation-resistant TDP-43 conformers to mitigate neurodegeneration
Article Snippet: .. Protein was then buffer exchanged into fluorescence assay buffer (150 mM NaCl, 20 mM HEPES-NaOH pH 7.0, 1 mM DTT) using BIO-RAD Micro Bio-Spin Chromatography Columns, following manufacturer’s instructions. ..
Chromatography:Article Title: Short RNA chaperones promote aggregation-resistant TDP-43 conformers to mitigate neurodegeneration
Article Snippet: .. Protein was then buffer exchanged into fluorescence assay buffer (150 mM NaCl, 20 mM HEPES-NaOH pH 7.0, 1 mM DTT) using BIO-RAD Micro Bio-Spin Chromatography Columns, following manufacturer’s instructions. ..
Article Title: Consequences of point mutations in melanoma-associated antigen 4 (MAGE-A4) protein: Insights from structural and biophysical studies.
Article Snippet: .. Prior to analysis, samples were thawed and dialyzed using Bio-RAD micro bio-spin chromatography columns (Bio-Rad Laboratories, Inc) into 50 mM Ammonium Acetate and diluted to 20 μ M. Protein concentrations were confirmed post dialysis spectrophotometrically (NanoDrop Spectrophotometer ND 1000, Thermo Scientific, USA). .. Mass Spectrometry experiments were performed on a Micro Mass Quadruple Time-of-Flight Ultima Global (Waters, Manchester, UK) and ion mobility mass spectrometry measurements were made on an in-house modified quadrupole time-of-flight mass spectrometer including a 5.1 cm drift cell44.
Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Article Title: Short RNA chaperones promote aggregation-resistant TDP-43 conformers to mitigate neurodegeneration
Article Snippet: .. For “free” conditions, protein was buffer exchanged into Non-Deuterated Buffer (150 mM NaCl, 20 mM HEPES-NaOH pH 7.0, 1 mM DTT) using BIO-RAD Micro Bio-Spin Chromatography Columns, following manufacturer’s instructions. ..
Article Title: Ubiquitin-conjugating enzyme UBE2N modulates proteostasis in immunoproteasome-positive acute myeloid leukemia
Article Snippet: .. Finally, the beads were isolated using Bio-Rad micro bio-spin chromatography columns (Bio-Rad, #7326204), and the samples were analyzed with SDS-PAGE and immunoblotting. ..
Article Title: Short RNA chaperones promote aggregation-resistant TDP-43 conformers to mitigate neurodegeneration
Article Snippet: .. Protein was then buffer exchanged into 150 mM NaCl, 20 mM HEPES-NaOH pH 7.0 (or pH 6.0 where indicated), 10% glycerol, 1 mM DTT using BIO-RAD Micro Bio-Spin Chromatography Columns, following manufacturer’s instructions. ..
Spectrophotometry:Article Title: Consequences of point mutations in melanoma-associated antigen 4 (MAGE-A4) protein: Insights from structural and biophysical studies.
Article Snippet: .. Prior to analysis, samples were thawed and dialyzed using Bio-RAD micro bio-spin chromatography columns (Bio-Rad Laboratories, Inc) into 50 mM Ammonium Acetate and diluted to 20 μ M. Protein concentrations were confirmed post dialysis spectrophotometrically (NanoDrop Spectrophotometer ND 1000, Thermo Scientific, USA). .. Mass Spectrometry experiments were performed on a Micro Mass Quadruple Time-of-Flight Ultima Global (Waters, Manchester, UK) and ion mobility mass spectrometry measurements were made on an in-house modified quadrupole time-of-flight mass spectrometer including a 5.1 cm drift cell44.
Buffer Exchange:Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Molecular Weight:Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Membrane:Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Mass Spectrometry:Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Software:Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Expressing:Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Purification:Article Title: Identifying key membrane protein lipid interactions using mass spectrometry
Article Snippet: .. × 0.58-mm i.d. (cat. no. GC100-10) Superdex 200 Increase 5/150 GL (GE Healthcare, cat. no. 28990945) Acclaim PepMap 100, C18, 75 μm × 15 cm (Thermo Fisher Scientific) Bio-Rad Micro Bio-Spin chromatography columns (Empty; Bio-Rad, cat. no. 732-6204) Buffer-exchange devices, Micro Bio-Spin 6 chromatography columns, MW exclusion limit 6 kDa (Tris buffer, Bio-Rad, cat. no. 732-6221) Centrifugal concentrators (molecular weight cutoff (MWCO) 50/100 kDa, Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-30-K membrane, Millipore, cat. no. UFC906096; NMWL Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-50 K, ultracel-100-K membranes) Centrifuge tubes with screw caps (Ultra High Performance, 15 ml, VWR, cat. no. 21008-214 and Ultra High Performance, 50 ml, VWR, 21008-240) AKTA Purifier (GE Healthcare) HisTrap HP column (1 ml; GE Healthcare, cat. no. 17-5247-01) SpeedVac Sonicator UHPLC: we used Dionex-3000 UHPLC for the LC-MS/MS analysis of the lipids Mass spectrometer: we used a Synapt G1 (Waters) mass spectrometer for high-energy nMS and an LTQ Orbitrap XL Hybrid Ion Trap–Orbitrap mass spectrometer (Thermo Fisher Scientific) for lipidomics MassLynx software version 4.1 (Waters): used to analyze MS data from Waters instrument Xcalibur (Thermo Fisher Scientific): used to analyze the lipidomics data and LC-MS/MS data (these last two software programs may come with the instrument, or can be purchased as stand-alone software from the respective vendors) REAGENT SETUP Membrane protein expression and purification Depending on the target membrane protein complex, we suggest the expression and purification strategies in previously published protocols7,26. ..
Isolation:Article Title: Ubiquitin-conjugating enzyme UBE2N modulates proteostasis in immunoproteasome-positive acute myeloid leukemia
Article Snippet: .. Finally, the beads were isolated using Bio-Rad micro bio-spin chromatography columns (Bio-Rad, #7326204), and the samples were analyzed with SDS-PAGE and immunoblotting. ..
SDS Page:Article Title: Ubiquitin-conjugating enzyme UBE2N modulates proteostasis in immunoproteasome-positive acute myeloid leukemia
Article Snippet: .. Finally, the beads were isolated using Bio-Rad micro bio-spin chromatography columns (Bio-Rad, #7326204), and the samples were analyzed with SDS-PAGE and immunoblotting. ..
Western Blot:Article Title: Ubiquitin-conjugating enzyme UBE2N modulates proteostasis in immunoproteasome-positive acute myeloid leukemia
Article Snippet: .. Finally, the beads were isolated using Bio-Rad micro bio-spin chromatography columns (Bio-Rad, #7326204), and the samples were analyzed with SDS-PAGE and immunoblotting. ..
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